The use of antisense therapy for the treatment of Alzheimers Disease

If you base your work on extending the work of that from the link below, there are some considerations to think about…

If you are going to include tau in your approach, you need a mouse model that exhibits the neurofibrillary tangles associated with dysregulated tau. Have you seen such mice in the literature?

Once/If you identify such mice, then you need to know the mRNA sequence for the tau protein so that you can design your ASO against it.

You will need to consider your method of ASO delivery… the intracerebral ventricular approach used by the researchers below may be valid. (Later on… toward the end of your discussion… you should address how this approach might be applied to humans for treatment.)

You will need a set of experiments showing in cultured neurons expressing B-amyloid and tau that your ASOs will inhibit their expression a great deal.

For the mouse model treatments, you will need a way to determine (perhaps postmortem) that the ASOs did indeed enter brain cells and inhibit B-amyloid and tau.

For behavioral analyses of the mice after treatment, this could be put into the ‘future directions’ aspect of your thesis.

The above roughly outlines the project. Keep in mind that you will be filling in with much detail. For each aspect of the project given above, be sure you contemplate and write about: why this aspect is being done? what information will it give you? how does that information fit into the current state of knowledge reflected in the literature? Remember to have ‘controls’ for your experiments and your procedures – you need to be able to justify that what you are doing (whether experiment or even simply a readout e.g. western blot) is real… that it worked… that differences among experimental groups are significant and not due to chance